The major focus in my laboratory is to study the role of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in regulating corpus luteum architecture and function. By utilizing northern and western blotting, zymography, in situ hybridization, immunohistochemistry, and cell culture techniques, we have been able to identify the presence of MMPs (gelatinases, membrane-type 1 and TIMPs (e.g., TIMP-1, TIMP-2, TIMP-3) in the cow corpus luteum over the estrous cycle. We have also shown that TIMP-1 and TIMP-2 proteins decrease dramatically in sheep corpus luteum following a one-hour infusion of prostaglandin F2a (PGF2a).
Recently, a second focus in my laboratory is to study age and growth, reproductive cyclicity and genetics of two skate species found in the Gulf of Maine. This information is vital to the development of a fisheries management plan for these two over-fished elasmobranchs.
Utilizing the technique of ultrasonography, we also participate in a regional project examining the regulation of follicle development in cattle.
Faculty Award Winner
Award Type: COLSA Outstanding Advisor Award Year: 2013
Ph.D., Biology, Boston University
B.A., Anatomy and Physiology, Cornell University
BCHM/BMS 802/702: Endocrinology
BMCB 605: Eukaryotic Cell & DevlpBio\Hon
BMCB 795W: Invest in Molecular & Cell Bio
BMCB 799H: Honors Senior Thesis
BMS 795W: Investigations Biomedical Sci
BMS 799H: Senior Honors Thesis
INCO 590: Rsrch Exp/MCBS
INCO 790: Advanced Research Experience
Prohaska, B. K., Tsang, P. C. W., III, D. W. B., Hoffmayer, E. R., Wheeler, C. R., & Sulikowski, J. A. (2018). Effects of delayed phlebotomy on plasma steroid hormone concentrations in two elasmobranch species. JOURNAL OF APPLIED ICHTHYOLOGY, 34(4), 861-866. doi:10.1111/jai.13700
Foradori, M. J., Chen, Q., Fernandez, C. A., Harper, J., Li, X., Tsang, P. C. W., . . . Moses, M. A. (2014). Matrilin-1 Is an Inhibitor of Neovascularization. JOURNAL OF BIOLOGICAL CHEMISTRY, 289(20), 14301-14309. doi:10.1074/jbc.M113.529982
Bubley, W. J., Sulikowski, J. A., Koester, D. M., & Tsang, P. C. W. (2013). Using a multi-parameter approach to reassess maturity of spiny dogfish, squalus acanthias, following increased fishing pressure in the western north atlantic. Fisheries Research, 147, 202-212. doi:10.1016/j.fishres.2013.06.004
Williams, L. J., Campbell, M. D., Tsang, P. C. W., & Sulikowski, J. A. (2013). Using estradiol and progesterone concentrations to assess individual variability in the reproductive cyclicity of captive female little skates, Leucoraja erinacea, from the western Gulf of Maine. Fish Physiology and Biochemistry, 39(5), 1089-1099. doi:10.1007/s10695-012-9766-2
Prohaska, B. K., Tsang, P. C. W., Driggers, W. B., Hoffmayer, E. R., & Sulikowski, J. A. (2013). Development of a Nonlethal and Minimally Invasive Protocol to Study Elasmobranch Reproduction. Marine and Coastal Fisheries, 5(1), 181-188. doi:10.1080/19425120.2013.788590
Townson, D. H., Tsang, P. C. W., Butler, W. R., Frajblat, M., Griel, L. C., Johnson, C. J., . . . Pate, J. L. (2002). Relationship of fertility to ovarian follicular waves before breeding in dairy cows. JOURNAL OF ANIMAL SCIENCE, 80(4), 1053-1058. Retrieved from http://gateway.webofknowledge.com/
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Hinshelwood, M. M., Corbin, C. J., Corbin, C. J., Tsang, P. C. W., Tsang, P. C. W., Simpson, E. R., & Simpson, E. R. (1993). Isolation and characterization of a complementary deoxyribonucleic acid insert encoding bovine aromatase cytochrome P450. Endocrinology, 133(5), 1971-1977.
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