Neurobiology of pain and anxiety, voltage-gated calcium channels, human genetics, comparative transcriptomics, neuropharmacology.
Description of Current Research:
Pain and anxiety are two highly prevalent neurological conditions in humans. Unfortunately, the currently available pharmacological treatments for these conditions have many undesirable side effects. The scarcity of specific treatments with a more favorable side effect profile drives my motivation to investigate the molecular mechanisms underlying pain and anxiety. In particular, I focus on the role of neuronal voltage-gated calcium channels and endocannabinoids in specific cell populations of the pain pathway and in brain areas implicated in anxiety-like behavior. Voltage-gated calcium channels are key players in the modulation of neuronal circuits associated with pain and anxiety. In addition, endocannabinoids are internal signaling molecules that relieve pain and anxiety, partially by inhibiting voltage-gated calcium channels. My goal is to understand the coupling of endocannabinoids to voltage-gated calcium channels in cell-specific neuronal populations during situations that induce anxiety or chronic pain. To achieve this goal, I use modern electrophysiological techniques coupled to optogenetics, unique genetic models, and behavior. In the long term, I am interested in finding new cell-specific targets to mitigate anxiety-like behavior and pain. To do this, I will compare whole-cell transcriptome across specific neuronal subpopulations that are highly linked to anxiety and pain. As part of extensive collaborations with human geneticists, I also study mutations in voltage-gated calcium channels that are linked to neurological disorders, including myoclonus dystonia and schizophrenia.
If you are interested in doing exciting research in an environment that values diversity and the provision of high-quality mentorship, please contact me.
Ph.D., National Polytechnic Institute of Toulouse
B.S., University of Michoacan
BIOL 805: Neurbiology
GEN 999: Doctoral Research
INCO 590: Rsrch Exp/Biological Sciences
INCO 790: Adv Rsrch Exp/Biology Sciences
NSB 500: Neuroscience and Behavior I
NSB 501: Neurobiology Laboratory I
ZOOL 777: Neurobiology and Behavior
Sandoval, A., Duran, P., Gandini, M. A., Andrade, A., Almanza, A., Kaja, S., & Felix, R. (2017). Regulation of L-type Ca V 1.3 channel activity and insulin secretion by the cGMP-PKG signaling pathway. Cell Calcium, 66, 1-9. doi:10.1016/j.ceca.2017.05.008
Allen, S. E., Toro, C. P., Andrade, A., López-Soto, E. J., Denome, S., & Lipscombe, D. (2017). Cell-Specific RNA Binding Protein Rbfox2 Regulates CaV2.2 mRNA Exon Composition and CaV2.2 Current Size.. eNeuro, 4(5). doi:10.1523/ENEURO.0332-16.2017
Andrade, A., Hope, J., Allen, A., Yorgan, V., Lipscombe, D., & Pan, J. Q. (2016). A rare schizophrenia risk variant of CACNA1I disrupts CaV3.3 channel activity. Scientific Reports, 6(1). doi:10.1038/srep34233
Lipscombe, D., & Andrade, A. (2015). Calcium channel CaVα1 splice isoforms-Tissue specificity and drug action. Current molecular pharmacology, 8(1), 22-31.
Groen, J. L., Andrade, A., Ritz, K., Jalalzadeh, H., Haagmans, M., Bradley, T. E. J., . . . Denome, S. (2014). CACNA1B mutation is linked to unique myoclonus-dystonia syndrome. Human molecular genetics, 24(4), 987-993.
Lipscombe, D., Andrade, A., & Allen, S. E. (2013). Alternative splicing: Functional diversity among voltage-gated calcium channels and behavioral consequences. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1828(7), 1522-1529. doi:10.1016/j.bbamem.2012.09.018
Marangoudakis, S., Andrade, A., Helton, T. D., Denome, S., Castiglioni, A. J., & Lipscombe, D. (2012). Differential Ubiquitination and Proteasome Regulation of CaV2.2 N-Type Channel Splice Isoforms. Journal of Neuroscience, 32(30), 10365-10369. doi:10.1523/jneurosci.0851-11.2012
Calderón-Rivera, A., Andrade, A., Hernández-Hernández, O., González-Ramírez, R., Sandoval, A., Rivera, M., . . . Felix, R. (2012). Identification of a disulfide bridge essential for structure and function of the voltage-gated Ca2+ channel α2δ-1 auxiliary subunit. Cell Calcium, 51(1), 22-30. doi:10.1016/j.ceca.2011.10.002
Andrade, A., Sandoval, A., González-Ramírez, R., Lipscombe, D., Campbell, K. P., & Felix, R. (2009). The α2δ subunit augments functional expression and modifies the pharmacology of CaV1.3 L-type channels. Cell Calcium, 46(4), 282-292. doi:10.1016/j.ceca.2009.08.006
Sandoval, A., Andrade, A., Beedle, A. M., Campbell, K. P., & Felix, R. (2007). Inhibition of Recombinant N-Type CaV Channels by the 2 Subunit Involves Unfolded Protein Response (UPR)-Dependent and UPR-Independent Mechanisms. Journal of Neuroscience, 27(12), 3317-3327. doi:10.1523/jneurosci.4566-06.2007