Anita S. Klein

ASSOCIATE PROFESSOR
Faculty Fellow
Phone: (603) 862-2809
Office: Biological Sciences, Spaulding Hall Rm 238, Durham, NH 03824
Anita Klein

My research activities have ranged from the biochemical to the genetic level and included soybean, maize genetics, conifer evolution and population genetics, and the population genetics and genomics of macroalgae. Most recently I have been a member the NSF Research Coordination Network for the annotation and application of the genome sequence of Porphyra umbilicalis Kutzing. With my former student Dr. Renee Eriksen and my current student Yuanyu (Helen) Cao, I am annotating genes for stress metabolism, nitrogen assimilation intermediary metabolism and carbohydrate synthesis. I am applying molecular tools and genetic selection to develop Porphyra cultivars with enhanced nutrient uptake and thermal tolerance Porphyra for integrated multi-trophic aquaculture. I have collaborated with several UNH Colleagues including Art Mathieson, Chris Neefus, Bob Eckert, Dave Burdick, Linn Bogle, Gregg Moore, Fred Short

Education

  • Ph.D., Biochemistry, Michigan State University
  • B.A., Biology, University of Rochester

Courses Taught

  • BIOL 544: Your Genes, Your Life
  • GEN 705: Population/Quant Genetics
  • GEN 999: Doctoral Research
  • INCO 590: Rsrch Exp/Biological Sciences
  • MCBS 999: Doctoral Thesis
  • PBIO 985: Advanced Topics

Selected Publications

Cao, Y., Green-Gavrielidis, L. A., Eriksen, R. L., & Klein, A. S. (2019). A pilot study of genetic structure of Porphyra umbilicalis Kützing in the Gulf of Maine using SNP markers from RNA-Seq. Journal of Applied Phycology, 31(2), 1493-1503. doi:10.1007/s10811-018-1604-1

Eriksen, R. L., & Klein, A. S. (2018). Organism-environment interactions and differential gene expression patterns among open-coastal and estuarine populations of Porphyra umbilicalis Kützing (Rhodophyta) in the Northwest Atlantic. Fisheries and Aquatic Sciences, 21(1). doi:10.1186/s41240-018-0103-2

Brawley, S. H., Blouin, N. A., Ficko-Blean, E., Wheeler, G. L., Lohr, M., Goodson, H. V., . . . Prochnik, S. E. (2017). Insights into the red algae and eukaryotic evolution from the genome ofPorphyra umbilicalis(Bangiophyceae, Rhodophyta). Proceedings of the National Academy of Sciences, 114(31), E6361-E6370. doi:10.1073/pnas.1703088114

Eriksen, R. L., Green, L. A., & Klein, A. S. (2016). Genetic variation within and among asexual populations of Porphyra umbilicalis Kützing (Bangiales, Rhodophyta) in the Gulf of Maine, USA. Botanica Marina, 59(1), 1-12. doi:10.1515/bot-2015-0017

Benton, C. S., Mathieson, A. C., & Klein, A. S. (2015). Ecology ofCodium fragileSubsp.FragilePopulations Within Salt Marsh Pannes in Southern Maine. Rhodora, 117(971), 297-316. doi:10.3119/14-14

Chan, C. X., Zäuner, S., Wheeler, G., Grossman, A. R., Prochnik, S. E., Blouin, N. A., . . . Bhattacharya, D. (2012). Analysis of Porphyra Membrane Transporters Demonstrates Gene Transfer among Photosynthetic Eukaryotes and Numerous Sodium-Coupled Transport Systems. Plant Physiology, 158(4), 2001-2012. doi:10.1104/pp.112.193896

Teasdale, B. W., & Klein, A. S. (2010). Genetic variation and biogeographical boundaries within the red alga Porphyra umbilicalis (Bangiales, Rhodophyta). Botanica Marina, 53(5). doi:10.1515/bot.2010.050

Gantt, E., Berg, G. M., Bhattacharya, D., Blouin, N. A., Brodie, J. A., Chan, C. X., . . . Brawley, S. H. (2010). Porphyra: Complex Life Histories in a Harsh Environment: P. umbilicalis, an Intertidal Red Alga for Genomic Analysis. In Cellular Origin, Life in Extreme Habitats and Astrobiology (pp. 129-148). Springer Netherlands. doi:10.1007/978-90-481-3795-4_7

Teasdale, B. W., West, A., Klein, A. S., & Mathieson, A. C. (2009). Distribution and evolution of variable group-I introns in the small ribosomal subunit of North AtlanticPorphyra(Bangiales, Rhodophyta). European Journal of Phycology, 44(2), 171-182. doi:10.1080/09670260802590877

Wallace, A. L., Klein, A. S., & Mathieson, A. C. (2006). A reply to Engel et al.. MOLECULAR ECOLOGY, 15(4), 1185-1187. doi:10.1111/j.1365-294X.2005.02759.x

Mathieson, A. C., Dawes, C. J., Wallace, A. L., & Klein, A. S. (2006). Distribution, morphology, and genetic affinities of dwarf embedded Fucus populations from the Northwest Atlantic Ocean. Botanica Marina, 49(4). doi:10.1515/bot.2006.036

West, A. L., Mathieson, A. C., Klein, A. S., Neefus, C. D., & Bray, T. L. (2005). Molecular ecological studies of New England species of<I> Porphyra</I> (Rhodophyta, Bangiales). Nova Hedwigia, 80(1), 1-24. doi:10.1127/0029-5035/2005/0080-0001

Wallace, A. L., Klein, A. S., & Mathieson, A. C. (2004). DETERMINING THE AFFINITIES OF SALT MARSH FUCOIDS USING MICROSATELLITE MARKERS: EVIDENCE OF HYBRIDIZATION AND INTROGRESSION BETWEEN TWO SPECIES OFFUCUS(PHAEOPHYTA) IN A MAINE ESTUARY. Journal of Phycology, 40(6), 1013-1027. doi:10.1111/j.1529-8817.2004.04085.x

Klein, A. S., Mathieson, A. C., Neefus, C. D., Cain, D. F., Taylor, H. A., Teasdale, B. W., . . . Wallace, A. L. (2003). Identification of north-western Atlantic Porphyra (Bangiaceae, Bangiales) based on sequence variation in nuclear SSU and plastid rbcL genes. Phycologia, 42(2), 109-122. doi:10.2216/i0031-8884-42-2-109.1

Li, J., Bogle, A. L., & Klein, A. S. (1999). Phylogenetic relationships in the Hamamelidaceae: Evidence from the nucleotide sequences of the plastid genematK. Plant Systematics and Evolution, 218(3-4), 205-219. doi:10.1007/bf01089228

Germano, J., & Klein, A. S. (1999). Species-specific nuclear and chloroplast single nucleotide polymorphisms to distinguish Picea glauca, P. mariana and P. rubens. Theoretical and Applied Genetics, 99(1-2), 37-49. doi:10.1007/s001220051206

Li, J., Bogle, A. L., & Klein, A. S. (1999). Phylogenetic relationships of the Hamamelidaceae inferred from sequences of internal transcribed spacers (ITS) of nuclear ribosomal DNA. American Journal of Botany, 86(7), 1027-1037. doi:10.2307/2656620

Camacho, F. J., Gernandt, D. S., Liston, A., Stone, J. K., & Klein, A. S. (1997). Endophytic fungal DNA, the source of contamination in spruce needle DNA. Molecular Ecology, 6(10), 983-987. doi:10.1046/j.1365-294x.1997.00266.x

Li, J. H., Bogle, A. L., & Klein, A. S. (1997). Interspecific relationships and genetic divergence of the disjunct genus Liquidambar (Hamamelidaceae) inferred from DNA sequences of plastid gene matK. RHODORA, 99(899), 229-240. Retrieved from http://gateway.webofknowledge.com/

Li, J. H., Bogle, A. L., & Klein, A. S. (1997). Phylogenetic relationships in the Corylopsis complex (Hamamelidaceae): Evidence from sequences of the internal transcribed spacers of nuclear ribosomal DNA and morphology. RHODORA, 99(900), 302-318. Retrieved from http://gateway.webofknowledge.com/

Bobola, M. S., Hillenberg, K. A., Gendreau, S. B., Eckert, R. T., Klein, A. S., & Stapelfeldt, K. (1996). Hybridization between Picearubens and Piceamariana: differences observed between montane and coastal island populations. Canadian Journal of Forest Research, 26(3), 444-452. doi:10.1139/x26-050

Bobola, M. S., Guenette, D., Eckert, R. T., Klein, A. S., Stapelfeldt, K., & Smith, D. E. (1996). Using nuclear and organelle DNA markers to discriminate among PicearubensPiceamariana, and their hybrids. Canadian Journal of Forest Research, 26(3), 433-443. doi:10.1139/x26-049

Smith, D. E., & Klein, A. S. (1994). Phylogenetic Inferences on the Relationship of North American and European Picea Species Based on Nuclear Ribosomal 18S Sequences and the Internal Transcribed Spacer 1 Region. Molecular Phylogenetics and Evolution, 3(1), 17-26. doi:10.1006/mpev.1994.1003

Bobola, M. S., Eckert, R. T., & Klein, A. S. (1992). Restriction fragment variation in the nuclear ribosomal DNA repeat unit within and between Picearubens and Piceamariana. Canadian Journal of Forest Research, 22(2), 255-263. doi:10.1139/x92-033

Dowe, M., Roman, G., & Klein, A. (1990). Excision and transposition of two Ds transposons from the bronze mutable 4 Derivative 6856 allele of Zea mays L.. MGG Molecular & General Genetics, 221(3). doi:10.1007/bf00259414

KLEIN, A. S., ROMAN, G., & PAJEMANALO, L. (1990). THE SHRUNKEN BRONZE-MUTABLE 4 ALLELIC FAMILY - CHARACTERIZATION OF A CHANGED STATE, SH BZ-M4 DERIVATIVE 6843B, OF THE ORIGINAL BZ-M4 MUTATION. MAYDICA, 35(4), 339-351. Retrieved from http://gateway.webofknowledge.com/

KLEIN, A. S., CLANCY, M., PAJEMANALO, L., FURTEK, D. B., HANNAH, L. C., & NELSON, O. E. (1988). THE MUTATION BRONZE-MUTABLE 4 DERIVATIVE 6856 IN MAIZE IS CAUSED BY THE INSERTION OF A NOVEL 6.7-KILOBASE PAIR TRANSPOSON IN THE UNTRANSLATED LEADER REGION OF THE BRONZE-1 GENE. GENETICS, 120(3), 779-790. Retrieved from http://gateway.webofknowledge.com/

Nelson, O. E., & Klein, A. S. (1984). CHARACTERIZATION OF AN &lt;em&gt;Spm&lt;/em&gt;-CONTROLLED BRONZE-MUTABLE ALLELE IN MAIZE. Genetics, 106(4), 769. Retrieved from http://www.genetics.org/content/106/4/769.abstract

Klein, A. S., & Nelson, O. E. (1983). Biochemical consequences of the insertion of a suppressor-mutator (&lt;em&gt;Spm&lt;/em&gt;) receptor at the bronze-1 locus in maize. Proceedings of the National Academy of Sciences, 80(24), 7591. Retrieved from http://www.pnas.org/content/80/24/7591.abstract

Klein, A. S., & Nelson, O. E. J. (1983). The allelic state at intensifier influences the accumulation of UDP glucose: flavonoid 3-O-glucosyltransferase in maize.. Phytochemistry, 22, 2634-2635.

Klein, A. S., Montezinos, D., & Delmer, D. P. (1981). Cellulose and 1,3 glucan synthesis during the early stages of wall regeneration in soybean protoplasts.. Planta, 152, 105-114.

Five major nuclear ribosomal repeats represent a large and variable fraction of the genomic DNA of Picea rubens and P. mariana. (n.d.). Molecular Biology and Evolution. doi:10.1093/oxfordjournals.molbev.a040702