Rick Cote

PROFESSOR
Phone: (603) 862-2458
Office: Molecular, Cellular, & Biomedical Sciences, Rudman Hall Rm 379, Durham, NH 03824
Cote

Faulty Award Winner

Award Type:  Distinguished Professor
Year:  2016

Education

  • Ph.D., Molecular Biology, University of Wisconsin
  • B.S., Biology-Chemistry, Tufts University

Research Interests

  • Biochemistry
  • Biochemistry, Proteins
  • Biomedical Research Resources, Other
  • Biomedical Research Training
  • Biomedical Research, Multidisciplinary
  • Biotechnology
  • Blindness
  • Catalysis/Kinetics
  • Cellular Physiology
  • Chromatography
  • Crop Protection (Pests, Diseases, & Weeds)
  • Drug Discovery
  • Enzymology
  • Eye Diseases
  • Gene Cloning
  • Molecular Biology
  • Molecular Cloning
  • Molecular Medicine
  • Nematology
  • Neurobiology
  • Neuropharmacology
  • Pharmacology
  • Preclinical Research
  • Proteins and Macromolecules
  • Proteomics
  • Receptors
  • Recombinant DNA
  • Research Training
  • Retinitis Pigmentosa
  • Science/Technology/Engineering/Math (STEM) Education
  • Scientific Instrumentation
  • Signal Transduction (Sensory/Cellular)
  • Structural Biology
  • University/Industrial Partnership
  • Vision
  • Vision Science

Courses Taught

  • BCHM 999: Doctoral Research
  • BENG 620: Tissue Engineering
  • INCO 590: Rsrch Exp/MCBS
  • MCBS 999: Doctoral Thesis
  • MESB 999: Doctoral Research

Selected Publications

Zeng-Elmore, X., Gao, X. -Z., Pellarin, R., Schneidman-Duhovny, D., Zhang, X. -J., Kozacka, K. A., . . . Chu, F. (2014). Molecular Architecture of Photoreceptor Phosphodiesterase Elucidated by Chemical Cross-Linking and Integrative Modeling. Journal of Molecular Biology, 426(22), 3713-3728. doi:10.1016/j.jmb.2014.07.033

Cahill, K. B., Quade, J. H., Carleton, K. L., & Cote, R. H. (2012). Identification of Amino Acid Residues Responsible for the Selectivity of Tadalafil Binding to Two Closely Related Phosphodiesterases, PDE5 and PDE6. Journal of Biological Chemistry, 287(49), 41406-41416. doi:10.1074/jbc.m112.389189

Zhang, X. -J., Gao, X. -Z., Yao, W., & Cote, R. H. (2012). Functional Mapping of Interacting Regions of the Photoreceptor Phosphodiesterase (PDE6) γ-Subunit with PDE6 Catalytic Dimer, Transducin, and Regulator of G-protein Signaling9–1 (RGS9–1). Journal of Biological Chemistry, 287(31), 26312-26320. doi:10.1074/jbc.m112.377333

Matte, S. L., Laue, T. M., & Cote, R. H. (2012). Characterization of Conformational Changes and Protein-Protein Interactions of Rod Photoreceptor Phosphodiesterase (PDE6). Journal of Biological Chemistry, 287(24), 20111-20121. doi:10.1074/jbc.m112.354647

Zhang, X. -J., Skiba, N. P., & Cote, R. H. (2010). Structural Requirements of the Photoreceptor Phosphodiesterase γ-Subunit for Inhibition of Rod PDE6 Holoenzyme and for Its Activation by Transducin. Journal of Biological Chemistry, 285(7), 4455-4463. doi:10.1074/jbc.m109.057406

Liu, Y. -T., Matte, S. L., Corbin, J. D., Francis, S. H., & Cote, R. H. (2009). Probing the Catalytic Sites and Activation Mechanism of Photoreceptor Phosphodiesterase Using Radiolabeled Phosphodiesterase Inhibitors. Journal of Biological Chemistry, 284(46), 31541-31547. doi:10.1074/jbc.m109.018606

Zhang, X. -J., Cahill, K. B., Elfenbein, A., Arshavsky, V. Y., & Cote, R. H. (2008). Direct Allosteric Regulation between the GAF Domain and Catalytic Domain of Photoreceptor Phosphodiesterase PDE6. Journal of Biological Chemistry, 283(44), 29699-29705. doi:10.1074/jbc.m803948200

Pentia, D. C., Hosier, S., & Cote, R. H. (2006). The Glutamic Acid-rich Protein-2 (GARP2) Is a High Affinity Rod Photoreceptor Phosphodiesterase (PDE6)-binding Protein That Modulates Its Catalytic Properties. Journal of Biological Chemistry, 281(9), 5500-5505. doi:10.1074/jbc.m507488200

Zhang, X., Feng, Q., & Cote, R. H. (2005). Efficacy and Selectivity of Phosphodiesterase-Targeted Drugs in Inhibiting Photoreceptor Phosphodiesterase (PDE6) in Retinal Photoreceptors. Investigative Opthalmology & Visual Science, 46(9), 3060. doi:10.1167/iovs.05-0257

Carleton, K. L., Spady, T. C., & Cote, R. H. (2005). Rod and Cone Opsin Families Differ in Spectral Tuning Domains but Not Signal Transducing Domains as Judged by Saturated Evolutionary Trace Analysis. Journal of Molecular Evolution, 61(1), 75-89. doi:10.1007/s00239-004-0289-z

Norton, A. W., Hosier, S., Terew, J. M., Li, N., Dhingra, A., Vardi, N., . . . Cote, R. H. (2005). Evaluation of the 17-kDa Prenyl-binding Protein as a Regulatory Protein for Phototransduction in Retinal Photoreceptors. Journal of Biological Chemistry, 280(2), 1248-1256. doi:10.1074/jbc.m410475200

Zhang, X. (2005). cGMP signaling in vertebrate retinal photoreceptor cells. Frontiers in Bioscience, 10(1-3), 1191. doi:10.2741/1612

Cote, R. H. (2004). Characteristics of Photoreceptor PDE (PDE6): similarities and differences to PDE5. International Journal of Impotence Research, 16(S1), S28-S33. doi:10.1038/sj.ijir.3901212

Paglia, M. J., Mou, H., & Cote, R. H. (2002). Regulation of Photoreceptor Phosphodiesterase (PDE6) by Phosphorylation of Its Inhibitory γ Subunit Re-evaluated. Journal of Biological Chemistry, 277(7), 5017-5023. doi:10.1074/jbc.m106328200

Mou, H., & Cote, R. H. (2001). The Catalytic and GAF Domains of the Rod cGMP Phosphodiesterase (PDE6) Heterodimer Are Regulated by Distinct Regions of Its Inhibitory γ Subunit. Journal of Biological Chemistry, 276(29), 27527-27534. doi:10.1074/jbc.m103316200

Tcheudji, J. F. K., Lebeau, L., Virmaux, N., Maftei, C. G., Cote, R. H., Lugnier, C., & Schultz, P. (2001). Molecular organization of bovine rod cGMP-phosphodiesterase 6. Journal of Molecular Biology, 310(4), 781-791. doi:10.1006/jmbi.2001.4813

Norton, A. W., D'Amours, M. R., Grazio, H. J., Hebert, T. L., & Cote, R. H. (2000). Mechanism of Transducin Activation of Frog Rod Photoreceptor Phosphodiesterase. Journal of Biological Chemistry, 275(49), 38611-38619. doi:10.1074/jbc.m004606200

Cote, R. X. (2000). [43] Kinetics and regulation of cGMP binding to noncatalytic binding sites on photoreceptor phosphodiesterase. Unknown Journal, 646-672. doi:10.1016/s0076-6879(00)15873-2

Mou, H., Grazio, H. J., Cook, T. A., Beavo, J. A., & Cote, R. H. (1999). cGMP Binding to Noncatalytic Sites on Mammalian Rod Photoreceptor Phosphodiesterase Is Regulated by Binding of Its γ and δ Subunits. Journal of Biological Chemistry, 274(26), 18813-18820. doi:10.1074/jbc.274.26.18813

D’AMOURS, M. R., & COTE, R. H. (1999). Regulation of photoreceptor phosphodiesterase catalysis by its non-catalytic cGMP-binding sites. Biochemical Journal, 340(3), 863. doi:10.1042/0264-6021:3400863

D'Amours, M. R., Granovsky, A. E., Artemyev, N. O., & Cote, R. H. (1999). Potency and mechanism of action of E4021, a type 5 phosphodiesterase isozyme-selective inhibitor, on the photoreceptor phosphodiesterase depend on the state of activation of the enzyme. MOLECULAR PHARMACOLOGY, 55(3), 508-514. Retrieved from http://gateway.webofknowledge.com/

Hebert, M. C., Schwede, F., Jastorff, B., & Cote, R. H. (1998). Structural Features of the Noncatalytic cGMP Binding Sites of Frog Photoreceptor Phosphodiesterase Using cGMP Analogs. Journal of Biological Chemistry, 273(10), 5557-5565. doi:10.1074/jbc.273.10.5557

Artemyev, N. O., Arshavsky, V. Y., & Cote, R. H. (1998). Photoreceptor Phosphodiesterase: Interaction of Inhibitory γ Subunit and Cyclic GMP with Specific Binding Sites on Catalytic Subunits. Methods, 14(1), 93-104. doi:10.1006/meth.1997.0568

Cote, R. H., Bownds, M. D., & Arshavsky, V. Y. (1994). cGMP binding sites on photoreceptor phosphodiesterase: role in feedback regulation of visual transduction.. Proceedings of the National Academy of Sciences, 91(11), 4845-4849. doi:10.1073/pnas.91.11.4845

COTE, R. H., & BRUNNOCK, M. A. (1993). INTRACELLULAR CGMP CONCENTRATION IN ROD PHOTORECEPTORS IS REGULATED BY BINDING TO HIGH AND MODERATE AFFINITY CGMP BINDING-SITES. JOURNAL OF BIOLOGICAL CHEMISTRY, 268(23), 17190-17198. Retrieved from http://gateway.webofknowledge.com/

COTE, R. H., NICOL, G. D., BURKE, S. A., & BOWNDS, M. D. (1989). CYCLIC-GMP LEVELS AND MEMBRANE CURRENT DURING ONSET, RECOVERY, AND LIGHT ADAPTATION OF THE PHOTORESPONSE OF DETACHED FROG PHOTORECEPTORS. JOURNAL OF BIOLOGICAL CHEMISTRY, 264(26), 15384-15391. Retrieved from http://gateway.webofknowledge.com/

Robinson, P. R., & Cote, R. H. (1989). Characterization of guanylate cyclase in squid photoreceptors. Visual Neuroscience, 3(01), 1-7. doi:10.1017/s0952523800012451

COTE, R. H., NICOL, G. D., BURKE, S. A., & BOWNDS, M. D. (1986). CHANGES IN CGMP CONCENTRATION CORRELATE WITH SOME, BUT NOT ALL, ASPECTS OF THE LIGHT-REGULATED CONDUCTANCE OF FROG ROD PHOTORECEPTORS. JOURNAL OF BIOLOGICAL CHEMISTRY, 261(28), 2965-2975. Retrieved from http://gateway.webofknowledge.com/

Cote, R. H., Nicol, G. D., Burke, S. A., & Bownds, M. D. (1986). Changes in cGMP concentration correlate with some, but not all, aspects of the light-regulated conductance of frog rod photoreceptors.. The Journal of biological chemistry, 261(28), 12965-12975.

ROBINSON, P. R., RADEKE, M. J., COTE, R. H., & BOWNDS, M. D. (1986). CGMP INFLUENCES GUANINE-NUCLEOTIDE BINDING TO FROG PHOTORECEPTOR G-PROTEIN. JOURNAL OF BIOLOGICAL CHEMISTRY, 261(1), 313-318. Retrieved from http://gateway.webofknowledge.com/

COTE, R. H., BIERNBAUM, M. S., NICOL, G. D., & BOWNDS, M. D. (1984). LIGHT-INDUCED DECREASES IN CGMP CONCENTRATION PRECEDE CHANGES IN MEMBRANE-PERMEABILITY IN FROG ROD PHOTORECEPTORS. JOURNAL OF BIOLOGICAL CHEMISTRY, 259(15), 9635-9641. Retrieved from http://gateway.webofknowledge.com/

Pentia, D. C., Hosier, S., Collupy, R. A., Valeriani, B. A., & Cote, R. H. (n.d.). Purification of PDE6 Isozymes From Mammalian Retina. Unknown Journal, 125-140. doi:10.1385/1-59259-839-0:125

Cote, R. H. (n.d.). Cyclic Guanosine 5'-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase. Unknown Journal, 141-154. doi:10.1385/1-59259-839-0:141