Biological Sciences: Integrative and Organismal Biological Sciences - PHD
Research Topic: Poplar gene regulation
By modulating the activity of polyamine pathway and over expressing the SPDS and mODC, we will study the growth of stem, leaves, roots, chlorophyll content and measure the dry carbon and nitrogen content and compare with the control cell lines. An additional goal is to study the effects of increased nitrogen use by the genetically manipulated plants on soil health, specifically the soil microbiome to ensure sustainable forest systems in the future with minimum damage to the environment. The specific objectives of my study would be production and characterization of transgenic poplar plants that will contain two transgene for high putrescine/ spermidine/ spermine; assimilation of effective nitrogen, effects of transgenic plants on soil microbiome and metabolomic studies via high-throughput computing approach. Previous studies in the transgenic cells showed elevated levels of mouse ODC enzyme activity, severalfold higher amounts of putrescine, a small increase in spermidine, and a small reduction in spermine as compared with NT cells.